An Overview of the Beneficial Effects of Hydrolysed Collagen as a Nutraceutical on Skin Properties: Scientific Background and Clinical Studies in The Open Nutraceuticals Journal, february 2015.

Lees de publicatie hier


The effect of oral collagen peptides supplementation on skin moisture and the dermal collagen network: evidence from an ex vivo model and randomized, placebo-controlled clinical trials


  • Jérome Asserin PhD, Elian Lati PhD, Toshiaki Shioya B.Eng., Janne Prawitt PhD

First published12 September 2015 Full publication history



Skin dryness and an accelerated fragmentation of the collagen network in the dermis are hallmarks of skin aging. Nutrition is a key factor influencing skin health and consequently its appearance. A wide range of dietary supplements is offered to improve skin health. Collagen peptides are used as a bioactive ingredient in nutricosmetic products and have been shown in preclinical studies to improve skin barrier function, to induce the synthesis of collagen and hyaluronic acid, and to promote fibroblast growth and migration. Our aim was to investigate the effect of oral supplementation with specific collagen peptides on skin hydration and the dermal collagen network in a clinical setting.


Two placebo-controlled clinical trials were run to assess the effect of a daily oral supplementation with collagen peptides on skin hydration by corneometry, on collagen density by high-resolution ultrasound and on collagen fragmentation by reflectance confocal microscopy. Human skin explants were used to study extracellular matrix components in the presence of collagen peptides ex vivo.


Oral collagen peptide supplementation significantly increased skin hydration after 8 weeks of intake. The collagen density in the dermis significantly increased and the fragmentation of the dermal collagen network significantly decreased already after 4 weeks of supplementation. Both effects persisted after 12 weeks. Ex vivo experiments demonstrated that collagen peptides induce collagen as well as glycosaminoglycan production, offering a mechanistic explanation for the observed clinical effects.


The oral supplementation with collagen peptides is efficacious to improve hallmarks of skin aging.





With a unique amino acid profile, ZZAZEN collagen peptides contain high levels of the amino acids glycine, hydroxyproline, proline, alanine and arginine providing specific nutritional benefits not found in other protein sources. In short, ZZAZEN offers multiple benefits linked to muscles, athlete performance and connective tissue support. This makes ZZAZEN great as sports nutrition.

ZZAZEN deliver numerous health and nutritional benefits to athletes both during and after exercise. The list of health benefits for sports nutrition:

  • Aiding muscular contraction
  • Muscle restoration
  • Provide energy for performance
  • Protecting joints and connective tissues
  • Weight management

Aiding muscular contraction

Muscular function is dependent on the energy produced by the breakdown of a compound called adenosine triphosphate (ATP) in cells. Creatine is a molecule consisting of three amino acids – glycine, arginine and methionine – that can aid muscular contraction during periods of high intensity exercise and also helps to replace depleted ATP levels. ZZAZEN, which consists of 20% glycine and 8% arginine, can support the synthesis of creatine in the body to improve performance during short bursts of exercise and help athletes to increase their body mass.

Muscle restoration

During and after long periods of exercise, protein loss can occur in the muscles as their structural integrity is compromised by oxidation, membrane microlesions and inflammatory reactions. A high protein sports nutrition diet just after exercise may enhance muscle anabolism, replacing lost proteins and helping to maintain and restore the protein content of the muscles while also reducing recovery time.

Providing energy for performance

Oral arginine supplements may also have a beneficial effect on athletic performance, even at a low dose. A daily dose of 10g of ZZAZEN produces the recommended dose of arginine per day. A study showed that by ingesting 1g supplements of arginine and ornithine taken five days per week for five weeks for sports nutrition can increase strength. Studies suggest that arginine stimulates the release of growth hormone from the pituitary gland, which may explain performance improvement.

Supporting connective tissues and preventing injuries

ZZAZEN can support joint health by regenerating cartilage and reducing joint inflammation, as demonstrated in a recent in-vivo study. ZZAZEN can increase the production of cartilage matrix, aggrecan and type II collagen.  An in vivo study also demonstrated a significant increase in collagen fibril diameter of the Achilles tendon after collagen peptides supplementation for 8 weeks.csm_musculoskeletal image_small_7d0b38c952

Collagen peptides may help protect the joint matrix and help to reduce joint discomfortA clinical study has shown that ZZAZEN can help reduce joint stiffness.

In a large-scale study of different categories of athletes, those taking a mix of collagen peptides, BCAA and arginine over a period of two years experienced a decreased tendon, ligament, joint and muscle injury rate. A smaller, placebo controlled study confirmed that consuming a collagen supplement with vitamin C, followed by a burst of intensive exercise, can help build ligaments, tendons and bones, and likely play a beneficial role in injury prevention and tissue repair. Additional clinical evidence for injury prevention are reported on athletes by Baar, 2015, and Ribas-Fernandez, 1998.

Weight management with sports nutrition

Known to be among the most satiating macronutrients, proteins can prevent cravings and help people reach their fitness and weight goals. ZZAZEN collagen peptides are particularly renowned for their ability to help individuals lose or maintain weight when incorporated in weight management formulations. csm_Peptan_Satiety_0ec49f9dc1

An in vivo study conducted in 2006 by the Laboratory of Physiology and Ingestive Behavior, INRA-AgroParis Tech (Paris, France), showed that ZZAZEN is more effective than a standard dairy protein in weight management.

A 2009 study demonstrated the improved satiating effect of collagen peptides on humans, in comparison to other proteins. The study showed that collagen peptides – taken alone or supplemented with tryptophan – are 30 to 50% more satiating than casein, soy or whey.

Source: Peptan

Main source of ZZAZEN is Peptan, worlds leader in collagen peptides.